The Effect of Extremely Low Frequency (elf) Pulsed Electromagnetic Field (pemf) on Collagenase Enzyme Kinetics* Uticaj Ekstremno Niskih Frekvencija (elf) Impulsnog Elektromagnetnog Polja Na Kinetiku Enzima Collagenase*

Current interest in the application of pulsed electromagnetic field (PEMF) as alternative therapy for different medical conditions has proved to be successful. A number of investigations demonstrated that low frequency PEMF enables the induction of significant current in tissues and allows for an enhanced biological effect and thus, can facilitate wound healing. In our previous study [1] we presented and discussed the design and development of an extremely low frequency (ELF) PEMF system that produces a time varying magnetic field of 0.5mT to 2.5mT the frequency range of 2-500Hz. 2-D and 3-D simulation results of the magnetic field produced by the system of two pairs of air core Helmholtz coils were also presented. In this study we present and discuss the application of the developed exposure system to study the effect of varying parametric changes of ELF PEMF exposures on Collagenase enzyme that plays a key role in wound healing. A comparative analysis of changes in the enzymatic activity of the irradiated vs. non-radiated Collagenase was performed for the selected frequencies of 2-500Hz and magnetic flux densities of 0.5-2.5mT. The results obtained reveal that the activity of a Collagenase enzyme can be modulated at the particular frequencies of ELF PEMF for magnetic flux densities between 0.5-2.5mT. The findings from this study can be used to determine the optimal characteristics of the applied ELF PEMF for wound treatment.